E. Leclerc, Y. Sakai, T. Fujii, Proc. MTAS2002, Nara, Japan, pp. 829-831, 2002

Description:

A PDMS microfluidic device composed of several microstructured layers is fabricated for perfusion culture of mammalian cells. Culturing cells up to higher cell density is one of the most important tasks to achieve physiologically meaningful functions, which are required for tissue engineering. In order to reach this goal, continuous nutrition and oxygen supply and waste removal through culture medium have to be ensured. In the conventional cell culture format using tissue culture treated dishes, large scale bioreactors, etc., however, it is quite difficult to facilitate the sufficient amount of circulation of those substances. Microfluidics enable us to realize microscale liquid conduits suitable for such kind of circulation system. Researchers have introduced a PDMS based microfluidic device for cell culture, which consists of multiple layers of PDMS with microfabricated structures. PDMS is a favorable material for this purpose because of its biocompatibility and high gas permeability. In the PDMS device, cells can be stably kept alive and oxygen can be supplied to the cells through the material itself. Moreover, by adopting PDMS as a material of the device, it is possible to fabricate complicated three-dimensional (3D) microstructures, which could maximize the surface area for cell attachment.

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