www.nanopicoftheday.org
July 5, 2004
Nanofiber Gene Delivery Chip
Source:
Timothy E.
McKnight
References:
"Tracking Gene Expression after DNA Delivery Using
Spatially Indexed Nanofiber Arrays" Timothy E.
McKnight, Anatoli V. Melechko, Dale K. Hensley, David G. J. Mann, Guy D.
Griffin, and Michael L. Simpson.
Nano Lett.; 2004; 4(7) pp 1213 - 1219
Description:
The penetration and residence of vertically aligned carbon nanofibers (VACNF)
within live cell matrices is demonstrated upon substrates that incorporate
spatially registered indices to facilitate temporal tracking of individual
cells. Penetration of DNA-modified carbon nanofibers into live cells using this
platform provides efficient delivery and expression of exogenous genes, similar
to "microinjection"-styled methods, but on a massively parallel basis. Spatially
registered indices on the substrate allow one to conveniently locate individual
cells, facilitating temporal tracking of gene expression events. This gene
delivery platform consists of arrays of individual carbon nanofibers at 5-um
pitch within numerically indexed, 100-um
square grid patterns. Fabrication of these devices on silicon substrates enables
mass production of 100 devices (5 mm2) per wafer, with each device
providing over 800,000 nanofiber-based "needles" for cellular impalement and
gene delivery applications.
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